National Institute on Aging, Developmental Genomics and Aging Section

National Institute on Aging
National Institutes of Health

Developmental Genomics and Aging Section

Minoru S.H. Ko, M.D., Ph.D., Chief
Senior Investigator

Overview: The long-term goal is to understand the fundamental mechanisms for the maintenance of self-renewal, immortality, and pluripotency of early mouse embryos and stem cells. Studies will utilize the potential of a systematic genomic approach - embryogenomics - to analyze global gene expression regulations. We believe that such global studies will provide greater understanding of mechanisms that will aid in the adaptation of stem cells to replacement therapy - regenerative medicine - for aging and dysfunctional cells and organs.

Projects

Systematic Analysis of Gene Regulatory Networks: studying gene regulatory networks in embryonic stem (ES) cells by systematically manipulating transcription factors and environmental conditions (NIA mouse ES cell bank). The project is also based on the resources developed by the NIA mouse cDNA project (completed).
Preimplantation Mouse Development: studying gene expression regulation in early embryos (from fertilization to implantation), which are the source of ES cells and the locus of nuclear reprogramming
Embryonic and Tissue Stem Cells: studying molecular similarities and differences between these stem cells

Resources

Downloads: scientific data related to our publications, microarray data sets, sequence data sets and annotations
Materials: NIA 15K and 7.4K mouse cDNA clone sets, DNA microarrays, mouse ES cells
Methods: laboratory protocols, including the ES cell manipulation and in situ hybridization

Software tools and databases >ALL

NIA Array Analysis: statistical analysis of microarray data
NIA Mouse Gene Index: databases of genes and alternative transcripts
CisFinder: identification of transcription factor-binding motifs from ChIP-seq data

Members

Positions

Publications >ALL

Nishiyama, Xin et al. Uncovering early response of gene regulatory networks in ES cells by systematic induction of transcription factors. Cell Stem Cell. 2009 Oct 2;5(4):420-33. [Abs]
Aiba, Nedorezov et al. Defining developmental potency and cell lineage trajectories by expression profiling of differentiating mouse embryonic stem cells. DNA Res. 2009 Feb;16(1):73-80. Epub 2008 Dec 26. [Abs]